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KMID : 0368420090520060630
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Journal of Plant Biology
2009 Volume.52 No. 6 p.630 ~ p.634
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Evaluation of Viability, Shedding Pattern, and Longevity of Pollen from Genetically Modified (GM) Herbicide-tolerant and Wild-type Zoysiagrass (Zoysia japonica Steud.)
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Kang Hong-Gyu
Bae Tae-Woong
Jeong Ok-Cheol
Sun Hyeon-Jin
Lim Pyung-Ok
Lee Hyo-Yeon
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Abstract
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Knowledge about pollen viability is important when evaluating the risk of genetically modified (GM) plants. Here, staining via iodine potassium iodide (IKI) or triphenyl tetrazolium chloride (TTC) could not distinguish between live and dead pollen from Zoysia japonica. Therefore, to obtain a reliable assessment of such viability and longevity, we developed an optimum germination medium containing 20% sucrose and 50 ppm H3BO3. Pollen grains transferred to the germination medium at about 1000 hours had a germination rate of >90%. Pollen was most predominantly shed at approximately 1000 hours, with viability declining to nearly 0% at 1200 hours. All germinability was lost within 150 min when stored at 25°C. No significant difference was found between GM and non-GM plants in their pollen viability or longevity.
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KEYWORD
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Gene flow, Genetically modified (GM), Pollen, Zoysia japonica
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